Michael Gale, Jr., Ph.D.

Michael Gale, Jr., Ph.D.

Professor, Immunology; Adjunct Professor, Microbiology & Global Health; Member, FHCRC/UW Cancer Consortium; Affiliate Investigator, Clinical Research Div.& Virus and Infectious Disease Div., FHCRC; Director, Center for Innate Immunity & Immune Disease

Dr. Michael Gale, Jr. received his training at the University of Washington School of Public Health and Community Medicine. He served on the faculty of the University of Texas Southwestern Medical Center until joining the University of Washington in 2007.

Contact Info

Department of Immunology
University of Washington  
Office E383, Box 358059
750 Republican Street
Seattle WA 981909-4766
Phone: 206-543-8514
Fax: 206-616-4274

Research Areas

  • Molecular Immunology
    Innate Immunity

LAB

Website for the Gale Laboratory

Center for Innate Immunity and Immune Disease (CIIID)

Pubmed

Michael Gale, Jr., Ph.D.

Research in the Gale laboratory is focused on understanding innate immunity to virus infection, and the intracellular immune processes and virus-host interactions that govern viral replication and infection outcome. The laboratory is a component of the Hepatitis C virus Cooperative Research Centers supported by the NIH. Additionally, the Gale laboratory has research programs focused on understanding immune control of West Nile virus infection, HIV infection, and the immunomodulatory/antiviral actions of interferons and small molecule inhibitors of virus replication.

Virus infection of mammalian cells triggers an intracellular immune response, termed the "innate immune response" that functions to suppress replication and spread of the virus. During infection specific motifs within viral products are recognized as pathogen associated molecular patterns (PAMPs) by cellular factors called pathogen recognition receptors (PRRs). Studies in the Gale laboratory have defined the retinoic acid-inducible gene I (RIG-I) as the major PRR that triggers immunity against hepatitis C virus and a variety of pathogenic RNA viruses. Accumulating evidence now indicates that immunity against RNA viruses is largely triggered through the PRR actions of RIG-I and/or a related protein called MDA5. RIG-I and MDA5 are cytosolic RNA helicase and are expressed at a low levels in most cells. During virus infection RIG-I or MDA5 bind to RNA PAMP motifs of viral genome or viral RNA replication products generated by specific viruses. RIG-I binding of viral RNA triggers its downstream signaling to induce the activation of latent transcription factors and the eventual production of alpha/beta interferons and expression of interferon-stimulated genes. These processes induce the innate immune response that serves to limit virus replication and spread. Many viruses direct actions of immune evasion through regulation of innate immune signaling and function. Our studies have linked the course of virus infection to regulation of innate immune processes, and have identified novel interactions as therapeutic targets for the intervention of infection.

 

1.  Wilkins, C., Woodward, J., Lau, D.T., Barnes, A., Joyce, M., McFarlane, N., McKeating, J.A., Tyrrell, D.L., Gale, M. Jr. (2013) IFITM1 is a tight junction protein that inhibits hepatitis C virus entry. Hepatology. 57:461-469. PMID: 22996292 PMCID: PMC3566288

2.  Wollish, A.C., Ferris, M.T., Blevins, L.K., Loo, Y-M., Gale, M. Jr. Heise, M.T. (2013) An attenuating mutation in a neurovirulent Sindbis virus strain interacts with the IPS-1 signaling pathway in vivo. Virology. 435:269-280. PMID: 23084425. PMCID: PMC3534923

3.  Lau, D.T., Negash, A., Chen, J., Crochet, N., Sinha, M., Zhang, Y., Guedj, J., Holder, S., Saito, T., Lemon, S.M., Luxon, B.A., Perelson, A.S., Gale, M. Jr. (2013) Innate immune tolerance and the role of kupffer cells in differential responses to interferon therapy among patients with HCV genotype 1 infection. Gastroenterology. 144:402-413. PMID: 23123437

4.  Mateo, R., Nagamine, C.M., Spagnolo, J., Mendez, E., Rahe, M., Gale, M. Jr., Yuan, J., Kirkegaard, K. (2013) Inhibition of cellular autophagy deranges dengue virion maturation. J Virol. 87:1312-1321. PMID: 23175363. PMCID: PMC3554187

5.  Ramos, H.J., Lanteri, M.C., Blahnik, G., Negash, A., Suthar, M.S., Brassil, M.M., Sodhi, K., Treuting, P.M., Busch, M.O., Norris, P.J., Gale, M. Jr. (2013) IL-1b signaling promotes CNS-intrinsic immune control of West Nile virus infection. PLoS Pathog. 8(11):e1003039. PMID: 23209411 PMCID: PMC3510243

6.  Lazear, H.M., Lancaster, A., Wilkins, C., Suthar, M.S., Huang, A., Vick, S.C., Clepper, L., Thackray, L., Brassil, M.M., Virgin, H.W., Nikolich-Zugich, J., Moses, A.V., Gale, M. Jr., Früh, K., Diamond, M.S. (2013) IRF-3, IRF-5, and IRF-7 Coordinately Regulate the Type I IFN Response in Myeloid Dendritic Cells Downstream of MAVS Signaling. PLoS Pathog. 9:e1003118. PMCID: PMC3536698

7.  Ma, D.Y., Suthar, M.S., Kasahara, S., Gale, M. Jr., Clark, E.A. (2013) CD22 is required for protection against West Nile Virus infection. J Virol. 87:3361-3375. PMCID: PMC3592166

8.  Kumar, M., Roe, K., Orillo, B., Muruve, D.A., Nerurkar, V.R., Gale, M. Jr., Verma, S. (2013) Inflammasome adaptor protein apoptosis-associated speck-like protein containing CARD (ASC) is critical for the immune response and survival in West Nile virus encephalitis. J Virol. 87:3655-3667 PMCID: PMC3624239

9.  Suthar, M.S., Diamond, M.S., Gale, M. Jr. (2013) West Nile virus infection and immunity. Nat Rev Microbiol. 11:115-128. PMID: 23321534

10.  Wilkins, C., Gale, M. Jr. (2013) Sterol-izing Innate Immunity. Immunity. 38:3-5. PMID: 23352217

11.  Ermler, M.E., Yerukhim, E., Schriewer, J., Schattgen, S., Traylor, Z., Wespiser, A.R., Caffrey, D.R., Chen, Z.J., King, C.H., Gale, M. Jr., Colonna, M., Fitzgerald, K.A., Buller, R.M., Hise, A.G. (2013) RNA helicase signaling is critical for type I interferon production and protection against Rift Valley Fever virus during mucosal challenge. J Virol.87:4846-4860. PMCID: PMC3624317

12.  Cho, H., Proll, S.C., Szretter, K.J., Katze, M.G., Gale, M. Jr., Diamond, M.S. (2013) Differential innate immune response programs in neuronal subtypes determine susceptibility to infection in the brain by positive-stranded RNA viruses. Nat Med 19:458-464. PMCID:3618596

13.   Suthar, M.S., Brassil, M.M., Blahnik, G., McMillan, A., Ramos, H.J., Proll, S.C., Belisle, S.E., Katze, M.G., Gale, M. Jr. (2013) A systems biology approach reveals that tissue tropism to West Nile virus is regulated by antiviral genes and innate immune cellular processes. PLoS Pathog. 9:e1003168. PMCID:3567171

14.   Negash, A.A., Ramos, H.J., Crochet, N., Lau, D.T., Doehle, B., Papic, N., Delker, D.A., Bertoletti, A., Hagedorn, C.H., Gale, M. Jr. (2013) IL-1β Production through the NLRP2 Inflammasome by Hepatic Macrophages Links Hepatitis C Virus Infection with Liver Inflammation and Disease. PLoS PAthog. 9:e1003330. PMCID: PMC3635973

15.   Stone, A.E., Giugliano, S., Schnell, G., Cheng, L., Leahy, K.F., Golden-Mason, L., Gale, M. Jr., Rosen, H.R. (2013) Hepatitis C Virus Pathogen Associated Molecular Pattern (PAMP) Triggers Production of Lambda-Interferons by Human Plasmacytoid Dendritic Cells. PLoS Pathog. 9:e1003316. PMCID: PMC3630164

16.   Li, J., Ding, S.C., Cho, H., Chung, B.C., Gale, M. Jr., Chanda, S.K., Diamond, M.S. (2013) A short hairpin RNA screen of interferon-stimulated genes identifies a novel negative regulator of the cellular antiviral response. MBio. 4(3):1-11. PMCID: PMC3684836

17.   Horner, S.M., Gale, M. Jr. (2013) Regulation of hepatic innate immunity by hepatitis C virus. Nat Med. 19(7):879-888. PMID: 23836238

18.   Lazear, H.M., Pinto, A.K., Ramos, H.J., Vick, S.C., Shrestha, B., Suthar, M.S., Gale, M. Jr., Diamond, M.S. (2013) The pattern recognition receptor MDA5 modulates CD8+ T cell-dependent clearance of West Nile virus from the central nervous system. J Virol. IN PRESS PMID: 23966390

19.  Errett, J.S., Suthar, M.S., McMillan, A., Diamond, M.S., Gale, M. Jr. (2013) The essential, non-redundant roles of RIG-I and MDA5 in detecting and controlling West Nile virus infection. J Virol. IN PRESS. PMID: 23966395

B.S., Zoology, University of Washington

Ph.D., Pathobiology, University of Washington

Michael Gale, Jr., PhD, Professor, PI

mgale@uw.edu

Yueh-Ming Loo, PhD, Research Assistant Professor

looy@uw.edu

 

 

GRADUATE STUDENTS

 

Lauren Aarreberg, Graduate student

laarrebe@uw.edu

Rebecca Olson, Graduate student

rmfritz@uw.edu

Andrey Shuvarikov, Graduate student

ashuvari@uw.edu

Kathryn Wuertz, Graduate student

kwuertz@uw.edu

 

 

POSTDOCTORAL FELLOWS

 

Kwan Chow, PhD, Postdoctoral Fellow

kwanchow@uw.edu

Katharina Esser-Nobis, PhD, Postdoctoral Fellow

ken10@uw.edu

Emily Hemann, PhD, Postdoctoral Fellow

eahemann@uw.edu

Alison Kell, PhD, Postdoctoral Fellow

kella@uw.edu

Sooyoung Lee, PhD, Postdoctoral Fellow

ssooyl@uw.edu

Amina Negash, PhD, Postdoctoral Fellow

negasha@uw.edu

Jen Rathe, MD Fellow

jarathe@uw.edu

Justin Roby, PhD, Postdoctoral Fellow

robyja@uw.edu

Amy Stone, PhD, Postdoctoral Fellow

stoneamy@uw.edu

 

 

LAB STAFF

 

Jackie Berhorst, Research Coordinator

jdao@uw.edu

Jean Chang, Research Scientist

mspiggy1@uw.edu

Nanette Crochet, Research Scientist

crochet@uw.edu

Michael Davis, Research Scientist

madphd@uw.edu

Megan De La Riva, Student Assistant

megandlr@uw.edu

Elyse Dewey, Research Scientist

edewey@uw.edu

Connor Driscoll, Data Analyst

driscolc@uw.edu

Marian Fairgrieve, Research Scientist

marianf@uw.edu

Frank Fernandez, IT Manager

fcf@uw.edu

Jenny Go, Research Scientist

tisoncik@uw.edu

Rich Green, Research Scientist

greener@uw.edu

Tien-Ying Hsiang, Research Scientist

tyhsiang@uw.edu

Renee Ireton, Research Scientist

rireton@uw.edu

Megan Knoll, Research Scientist

knollm@uw.edu

Lynn Law, Research Scientist

gllaw@uw.edu

Michelle Noval, Research Scientist

novalm@uw.edu

Aimee Sekine, Research Scientist

aimeem@uw.edu

Elise Smith, Research Scientist

eliser2@uw.edu

Sunil Thomas, Research Scientist

calvinho@uw.edu

Kathleen Voss, Research Scientist

katvoss@uw.edu